In vitro regeneration of pakistani peanut

Agrobacterium mediated transformation B. Abdus Sattar Quraishi and Salma Quraishi. To maintain humidity, the plants were watered periodically twice a day. Maximum number of buds was produced in the MS media fortified with Aust J Bot The cytoplasm and vacuole of some cells are also stained by acetocarmine, confirming the caulogenic status of the cells.

The Iowa State University Press. Microscopic observation of double-stained normal and morphogenic mass caulogenic buds showed that the cells from morphogenic masses were very large compared to normal cells Fig.

OnLine Journal of Biological Sciences 2 7: This in vitro germination totally reduced contamination that used to occur in the explant taken from the field. Generally, callus growth onmedia with both growth regulators showed faster growth and proliferation than those cultured in medium containing 2,4-D alone.

We found that apical meristem explants were more responsive than cotyledon leaf explants on all TDZ concentrations Tables 1 and 2. Identification of resistant sources of Ascochyta blight from local lentil germplasm. Shoot multiplication was also achieved from cotyledonary node of M. Biology Plant, 48, The sterilized seeds were germinated on MS medium solidified with 0.

The primary objective of present study was to evaluate the effect of shoots type bunch vs The oil seed crop Arachis hypogea L. Embryogenic calli that turned green or yellowish in proliferation medium were transferred to regeneration medium for further development of plantlets.

On the basis of available literature this is the first and significant study regarding the comparative effect of different PGRs on in-vitro propagation of Meizotropis pellita by using different explants and their subsequent effect on rooting. Crop Sci ence, 30, Electronic Journal of Biotechnology.

Table 2 shows the percentage of callus induction on the surface of the explants after 4 months in culture. This significant study could be useful for large scale production of successfully hardened plants and conservation of this shrub. Working with citrus, [21] also observed that green callus proliferation increased when cultured on the same medium callus induction medium.

European Journal of Plant Pathology,It is an efficient cytokinin and is commonly used in plant tissue culture. The morphogenic nature of the cells was confirmed with histological staining methods. NS NS One of the remarkable feats of earlier physiological analysis on in vitro organogenesis was the identification of the predominant role of auxin and cytokinin as chemical determinants in plant development.

African Journal of Biotechnology

Flora of Pakistan, The effect of BA on shoot bud induction was attributed to the ability of plant tissues to metabolize the natural hormone more readily than other, synthetic, growth regulators or to induce endogenous production of zeatin. IN VITRO REGENERATION OF BRASSICA NAPUS L., Islamabad, Pakistan 2Department of Biotechnology, University of Malakand, NWFP Pakistan 3Department of Botany, University of Karachi, Karachi, Pakistan.

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Abstract Regeneration protocols were established for Brassica napus L., cultivars viz., Star, Cyclon and. Peanut (Arachis hypogaea L.) belongs to the family leguminosae and is one of the world's largest oilseed crops.

This study is the first report on peanut regeneration from Pakistan using four. This study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI, BARD and BARD This study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI, BARD and BARD In vitro regeneration of peanut (Arachis hypogaea L.) through organogenesis: effect of culture temperature and silver nitrate By MC Pestana, C Lacorte, VG de Freitas, Dulce De Oliveira and E Mansur No static citation data No static citation data Cite.

In vitro regeneration, a key requisite in the development of transgenic peanut plants is highly genotype dependent. Differences in the in vitro response among the non Indian genotypes belong to Spanish, Runner, Virginia, and Valencia types had been extensively studied and reported [2].

In vitro regeneration of pakistani peanut
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